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	Provedor de dados BJM (2) Autor Culhari,Vitoria V.P. (1) Fukushima,Kazutaka (1) Ge,Yi Ping (1) Kelly,Steven L. (1) Liu,Wei Da (1) Lu,Gui Xia (1) Manning,Nigel (1) Melo,Nadja Rodrigues de (1) Miyaji,Makoto (1) Sano,Ayako (1) Shen,Yong Nian (1) Taguchi,Hideaki (1) Vilela,Marluce S. (1) Wang,Le (1) Mais... Palavra-chave Candida dubliniensis (2) Antifungal agents (1) Candida albicans (1) Differentiation (1) Identification (1) PCR-restriction fragment length polymorphism (1) Sterol composition (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2006 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A simple and reliable PCR-restriction fragment length polymorphism assay to identify Candida albicans and its closely related Candida dubliniensis BJM Ge,Yi Ping; Wang,Le; Lu,Gui Xia; Shen,Yong Nian; Liu,Wei Da. Candida dubliniensis is an emerging pathogen capable of causing superficial as well as systemic infections. Due to its close similarity to C. albcians, conventional methods based on phenotypic traits are not always reliable in identification of C. dubliniensis. In this study, we developed a PCR-restriction fragment length polymorphism (RFLP) assay to identify and discriminate between the two closely related species. The D1/D2 region of 28S rDNA was amplified by PCR and enzymatically digested by ApaI and BsiEI respectively. PCR products of both species were digested into two fragments by ApaI, but those of other yeast species were undigested. BsiEI cut the PCR products of C. albicans into two fragments but not those of C. dubliniensis. Thus two species were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Candida dubliniensis; PCR-restriction fragment length polymorphism; Identification; Differentiation. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300004 Candida dubliniensis in a Brazilian family with an HIV 1- infected child: identification, antifungal susceptibility, drug accumulation and sterol composition BJM Melo,Nadja Rodrigues de; Taguchi,Hideaki; Culhari,Vitoria V.P.; Sano,Ayako; Fukushima,Kazutaka; Miyaji,Makoto; Manning,Nigel; Kelly,Steven L.; Vilela,Marluce S.. This study investigated the prevalence of C. dubliniensis in a Brazilian family with an HIV - infected child. A total of 42 oral isolates were obtained from eight family members. The identification of C. dubliniensis was performed by polymerase chain reactions (PCR) using primers against a specific sequence of the C. dubliniensis cytochrome b gene. Only the HIV-infected child and his grandmother were colonized by C. dubliniensis. In this study C. dubliniensis isolated from the HIV-infected child exhibited high susceptibility for azoles tested with MICs of 0.125 and 0.5 µg/mL for voriconazole and fluconazole, respectively. Accumulation of [³H] fluconazole in C. dubliniensis isolated from the HIV-infected child was slightly reduced in comparison to the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida dubliniensis; Antifungal agents; Sterol composition. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300008 Registros recuperados: 2 Primeira ... 1 ... Última

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